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基因数据处理21之BWASW算法ref分块建立索引然后比对(ref切分为

发布时间:2021-05-18 08:15:43 所属栏目:大数据 来源:网络整理
导读:1.时间分析 对ref为单条染色体进行比对,第一次比对在3-5s不等,对chr1-4比对,在20s左右 连续比对多次后,对单染色体比对降到1s左右,chr1-4降到2s左右 不懂为什么比一次比对时间比较长,后面几次比对时间变短 运行代码: hadoop@Master:~/cloud/adam/xubo/

hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub$ samtools flagstat SRR003161h1000chr1.sam 
264 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 secondary
0 + 0 supplementary
0 + 0 duplicates
105 + 0 mapped (39.77% : N/A)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (N/A : N/A)
0 + 0 with itself and mate mapped
0 + 0 singletons (N/A : N/A)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)
hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub$ samtools flagstat SRR003161h1000chr2.sam 
260 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 secondary
0 + 0 supplementary
0 + 0 duplicates
83 + 0 mapped (31.92% : N/A)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (N/A : N/A)
0 + 0 with itself and mate mapped
0 + 0 singletons (N/A : N/A)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)
hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub$ samtools flagstat SRR003161h1000chr3.sam 
256 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 secondary
0 + 0 supplementary
0 + 0 duplicates
80 + 0 mapped (31.25% : N/A)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (N/A : N/A)
0 + 0 with itself and mate mapped
0 + 0 singletons (N/A : N/A)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)
hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub$ samtools flagstat SRR003161h1000chr4.sam 
254 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 secondary
0 + 0 supplementary
0 + 0 duplicates
58 + 0 mapped (22.83% : N/A)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (N/A : N/A)
0 + 0 with itself and mate mapped
0 + 0 singletons (N/A : N/A)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)
hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub$ samtools flagstat SRR003161h1000chr1-4.sam 
264 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 secondary
0 + 0 supplementary
0 + 0 duplicates
146 + 0 mapped (55.30% : N/A)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (N/A : N/A)
0 + 0 with itself and mate mapped
0 + 0 singletons (N/A : N/A)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)
3.比对结果文件,太长,就不粘了

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